1. First steps - Say Goodbye to the 🐭 ( mostly)¶

Lesson Objectives

Navigate your file system using the command line.
Access and read help files for bash programs and use help files to identify useful command options.
Demonstrate the use of tab completion, and explain its advantages.

questions

What is a command shell and why would I use one?
How can I move around on my computer?
How can I see what files and directories I have?
How can I specify the location of a file or directory on my computer?

How to access the shell¶

On a Mac or Linux machine, you can access a shell through a program called "Terminal", which is already available on your computer. The Terminal is a window into which we will type commands. If you're using Windows, you'll need to download a separate program to access the shell.

To save time, we are going to be working on a remote server where all the necessary data and software available. When we say a 'remote server', we are talking about a computer that is not the one you are working on right now.

Navigating your file system¶

The part of the operating system that manages files and directories is called the file system. It organizes our data into files, which hold information, and directories (also called "folders"), which hold files or other directories.

Several commands are frequently used to create, inspect, rename, and delete files and directories.

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The dollar sign is a prompt, which shows us that the shell is waiting for input; your shell may use a different character as a prompt and may add information before the prompt. When typing commands, either from these lessons or from other sources, do not type the prompt, only the commands that follow it.

Let's find out where we are by running a command called pwd (which stands for "print working directory"). At any moment, our current working directory is our current default directory, i.e., the directory that the computer assumes we want to run commands in, unless we explicitly specify something else. Here, the computer's response is /home/<username>, which is the top level directory within NeSI:

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$ pwd

/home/<username>

Let's look at how our file system is organized. We can see what files and subdirectories are in this directory by running ls, which stands for "listing":

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$ ls

shell_data

ls prints the names of the files and directories in the current directory in alphabetical order, arranged neatly into columns. We'll be working within the shell_data subdirectory, and creating new subdirectories, throughout this workshop.

The command to change locations in our file system is cd, followed by a directory name to change our working directory. cd stands for "change directory".

Let's say we want to navigate to the shell_data directory we saw above. We can use the following command to get there:

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cd shell_data

and take a look

$ ls

sra_metadata  untrimmed_fastq

We can make the ls output more comprehensible by using the flag -F, which tells ls to add a trailing / to the names of directories:

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$ ls -F

sra_metadata/  untrimmed_fastq/

what is /

Anything with a / after it is a directory. Things with a "*" after them are programs. If there are no decorations, it's a file.

ls has lots of other options. To find out what they are, we can type:

$ man ls

man (short for manual) displays detailed documentation (also referred as man page or man file) for bash commands. It is a powerful resource to explore bash commands, understand their usage and flags. Some manual files are very long. You can scroll through the file using your keyboard's down arrow or use the Space key to go forward one page and the b key to go backwards one page. When you are done reading, hit q to quit.

Busy looking terminal ? time to 🆑

Type the word clear into the terminal and press the Enter key.

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$ clear

This will scroll your screen down to give you a fresh screen and will make it easier to read. You haven't lost any of the information on your screen. If you scroll up, you can see everything that has been output to your screen up until this point.

Hot-key combinations are shortcuts for performing common commands.

The hot-key combination for clearing the console is Ctrl+L. Feel free to try it and see for yourself.

Challenge

Use the -l option for the ls command to display more information for each item in the directory. What is one piece of additional information this long format gives you that you don't see with the bare ls command?

solution

$ ls -l

total 8
drwxr-x--- 2 training training 4096 Jul 30  2015 sra_metadata
drwxr-xr-x 2 training training 4096 Nov 15  2017 untrimmed_fastq

The additional information given includes the name of the owner of the file, when the file was last modified, and whether the current user has permission to read and write to the file.

No one can possibly learn all of these arguments, that's what the manual page is for. You can (and should) refer to the manual page or other help files as needed.

Let's go into the untrimmed_fastq directory and see what is in there.

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$ cd untrimmed_fastq
$ ls -F

SRR097977.fastq  SRR098026.fastq

This directory contains two files with .fastq extensions. FASTQ is a format for storing information about sequencing reads and their quality. We will be learning more about FASTQ files in a later lesson.

Shortcut: Tab Completion¶

Typing out file or directory names can waste a lot of time and it's easy to make typing mistakes. Instead we can use tab complete as a shortcut. When you start typing out the name of a directory or file, then hit the Tab key, the shell will try to fill in the rest of the directory or file name.

Return to your home directory:

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$ cd

then enter:

$ cd she<tab>

The shell will fill in the rest of the directory name for shell_data.

Now change directories to untrimmed_fastq in shell_data

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$ cd shell_data
$ cd untrimmed_fastq

Using tab complete can be very helpful. However, it will only autocomplete a file or directory name if you've typed enough characters to provide a unique identifier for the file or directory you are trying to access.

For example, if we now try to list the files which names start with SR by using tab complete:

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$ ls SR<tab>

The shell auto-completes your command to SRR09, because all file names in the directory begin with this prefix. When you hit Tab again, the shell will list the possible choices.

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$ ls SRR09<tab><tab>

SRR097977.fastq  SRR098026.fastq

Tab completion can also fill in the names of programs, which can be useful if you remember the beginning of a program name.

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$ pw<tab><tab>

pwck      pwconv    pwd       pwdx      pwunconv

Displays the name of every program that starts with pw.

Summary

We now know how to move around our file system using the command line. This gives us an advantage over interacting with the file system through a GUI as it allows us to work on a remote server, carry out the same set of operations on a large number of files quickly, and opens up many opportunities for using bioinformatic software that is only available in command line versions.

In the next few episodes, we'll be expanding on these skills and seeing how using the command line shell enables us to make our workflow more efficient and reproducible.

The shell gives you the ability to work more efficiently by using keyboard commands rather than a GUI.
Useful commands for navigating your file system include: ls, pwd, and cd.
Most commands take options (flags) which begin with a -.
Tab completion can reduce errors from mistyping and make work more efficient in the shell.